Fig. 4. Interaction between MUC1 and mp120 ARM domain deletion constructs.
Lysates of (A) control S2-013.Neo and (B) MUC1 overexpressing S2-013.MUC1F that expressed mp120 with the indicated ARM domain deleted (ΔARM1-10) were immunoprecipitated with mAb 8D11 or mouse IgG control. Immunoprecipitates were split into two equal fractions: one was resolved on an 8% Tris-Glycine gel to detect mp120 by western blotting, and one was resolved on a 14% Tris-Glycine gel to detect MUC1 CT by western blotting.