Fig. 7. Orthotopic implantation of tumor cells in athymic nude mice.

(A) Tumor volume following orthotopic implantation of S2-013 cell lines re-expressing different p120 catenin isoforms with and without overexpression of MUC1. S2-013 cells (2.5×10⁵) re-expressing the indicated different p120 catenin isoforms with and without overexpression of MUC1 were injected into the pancreas of nude mice and allowed to grow for 30 days, at which time the animals were sacrificed and tumor volumes are quantified. (B) Orthotopic implantation of S2-013 cells with reexpression of p120 ctn 3A in the context of MUC1 overexpression exhibit liver metastasis. (C) H&E staining of formalin-fixed paraffin-embedded sections of tumors from orthotopically implanted S2-013 cells with and without expression of MUC1 and different p120 catenin isoforms.