Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Comp Neurol. 2014 Apr 12;522(11):2465–2480. doi: 10.1002/cne.23583

Figure 7. Aberrant LRRK2 Distribution Caused by Expression of Human LRRK2 BAC Constructs in Rats.

Figure 7

A) Western blot analysis with antibody N241 demonstrating the level of LRRK2 overexpression in the indicated brain region, as dissected from fresh rat brain tissue from either nontransgenic or LRRK2-G2019S human-BAC transgenic rats. Equivalent protein concentrations determined by BCA assay were loaded between lanes (and verified by actin signal), matched by region, between wild-type and transgenic rats. B) Forebrain lysate from nontransgenic rats was loaded in lane 1 and increasing concentrations of LRRK2-G2019S transgenic rat forebrain lysates were loaded in subsequent lanes. LRRK2 is overexpressed between 25 and 50 fold in the transgenic rats. C)The pan-reactive LRRK2 antibody N241A/34 was used to detect LRRK2 in LRRK2-G2019S hBAC positive rats in the motor cortex (scale bar is 50 μm), and D) SNpc, by fluorescent co-label with TH (scale bar is 0.1 mm). E) A striosome in the anterior striatum of transgenic rats identified by LRRK2, with intensely positive LRRK2 interneurons inside the striosome (scale bar is 50 μm. F,G) Co-localization of LRRK2 with cholinergic interneurons, but weak expression in parvalbumin-positive interneurons (scale bars are 10 μm). A magenta-green version of the images is available as Supplemental Figure 5.