Figure 10. Staining to confirm endothelial identity of pregnant sheep uterine artery endothelial cells (UAECs).

Endothelial identity was confirmed by (A) anti-vWF staining; (B and C) cobble-stone shaped appearance following staining with anti-β-catenin and anti-VE-cadherin respectively; (D) uptake of fluorescently labeled Ac-LDL. (E) is a representative negative control wherein the addition of the primary antibody was omitted. Scale bar = 100 µm (A, D and E); 50 µm (B and C).