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. 2014 Jun 30;9(6):e100021. doi: 10.1371/journal.pone.0100021

Table 2. Antibodies used to confirm identity of endothelial cells isolated from pregnant sheep uterine arteries.

Primary Antibody Secondary Antibody
polyclonal rabbit anti-human vWF (1∶400, A0082, Dako, Glostrup, Germany) Alexafluor-488 donkey anti-rabbit IgG (1∶1000, A21206, Invitrogen, Paisley, UK)
rabbit monoclonal anti- β-catenin (1∶2000, C2206, Sigma Aldrich, Gillingham, Dorset, UK) Alexafluor-488 donkey anti-rabbit IgG (1∶1000, A21206, Invitrogen, Paisley, UK)
mouse monoclonal anti-VE cadherin (1∶500, sc9989, Santa Cruz Biotechnology, Heidelberg, Germany) Alexafluor-488 goat anti-mouse IgG(1∶1000, A11001, Invitrogen, Paisley,UK)