Table 2. Antibodies used to confirm identity of endothelial cells isolated from pregnant sheep uterine arteries.
Primary Antibody | Secondary Antibody |
polyclonal rabbit anti-human vWF (1∶400, A0082, Dako, Glostrup, Germany) | Alexafluor-488 donkey anti-rabbit IgG (1∶1000, A21206, Invitrogen, Paisley, UK) |
rabbit monoclonal anti- β-catenin (1∶2000, C2206, Sigma Aldrich, Gillingham, Dorset, UK) | Alexafluor-488 donkey anti-rabbit IgG (1∶1000, A21206, Invitrogen, Paisley, UK) |
mouse monoclonal anti-VE cadherin (1∶500, sc9989, Santa Cruz Biotechnology, Heidelberg, Germany) | Alexafluor-488 goat anti-mouse IgG(1∶1000, A11001, Invitrogen, Paisley,UK) |