Figure 1. NEMO deficiency in recipient mice favours donor cell selection.

(A) Hepatocyte transplantation was applied to NEMO^Δhepa and WT recipient mice with preceding BMT. Donor cells were derived from WT (Casp8^loxP/loxP/hAAT(+)) or Caspase-8-deficient (Casp8^Δhepa/hAAT(+)) mice, respectively. (B) Serum hAAT levels were analysed via quantitative ELISA and displayed on a logarithmic scale. Basic values represent serum hAAT level 1d post transplantation and are used as a reference value for the calculation of the relative increase in the amount of donor derived hepatocytes over time. (C) Quantitative evaluation of hAAT (+) liver tissue areas 52 weeks after HT. (D) Visualization of engrafting donor cells using hAAT immunofluorescence staining. Clusters of donor derived hepatocytes are displayed 12 and 52 weeks after HT (blue: DAPI; red: hAAT(+) cells).