Figure 3. Reduced fibrogenesis in hepatocyte transplanted NEMO^Δhepa mice.

(A) Analysis of Collagen accumulation in transplanted mice via Sirius red staining under polarized light. NEMO^Δhepa mice that underwent transplantation with either Casp8^loxP/loxP/hAAT(+) or Casp8^Δhepa/hAAT(+) donor cells 52 weeks after HT were compared to age-matched completely untreated control mice as well as to bone marrow-transplanted mice 56 weeks (age matched) after BMT. (B) Collagen-1α staining for visualisation of a specific fibrotic collagen subtype (blue: DAPI: red/yellow: Collagen1-α). (C) Assessment of Collagen-1α mRNA expression via quantitative realtime PCR. (**p<0.01, ***p<0.001)