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. 2014 Jun 30;9(6):e100581. doi: 10.1371/journal.pone.0100581

Figure 4. The expression of miR-31 was examined in primary lung tumors (20 adenocarcinomas (ADC), 9 squamous cell carcinoma (SQC), and 10 large cell carcinomas (LCC), which comprised some of the 127 non-small cell lung cancers listed in Table 2, and 2 small cell carcinomas (SCC)).

Figure 4

MiR-31 levels were evaluated in primary lung tumors and the corresponding lung non-tumorous tissue. The copy numbers of miR-31 and U6 snRNA were measured using quantitative RT-PCR. Technical replicates were performed in triplicate. The means and standard deviations (error bars) of miR-31 levels normalized to those of U6 snRNA are shown as the results obtained from all tumors (A) and those of ADC (B). Significant differences in the mean miR-31 levels in adenocarcinomas among the different histological grades (9 well differentiated carcinomas (WEL); 3 moderately differentiated carcinomas (MOD); 8 poorly differentiated carcinomas (POR)) and subtypes (8 bronchioloalveolar carcinoma (BAC); 3 acinar carcinoma (ACN); 8 solid carcinoma (SOL)(one papillary carcinoma ( =  well differentiate carcinoma) was excluded from a statistical analysis) were analyzed with a one-way ANOVA. The means and standard deviations (error bars) of the histological grades (C) and subtypes (D) are presented.