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. 2014 Jun 21;14:461. doi: 10.1186/1471-2407-14-461

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effects of KIF2A silencing on cell proliferation, migration and invasion. The cell proliferation rate at 12, 24 and 36 hours, respectively, was inhibited in KIF2A-siRNA transfected cells (A). The numbers of migrating (B) and invasive (C) cells in the mock and nonsense-siRNA groups were significantly higher than those in the KIF2A-siRNA transfected group (P < 0.001). The data are representative of three experiments. Microscope images (with EOSIN staining, 200×) showing the mock, the nonsense-siRNA and the KIF2A-siRNA transfected cells in the migratory assay (D-F), and in the invasive assay (G-I).