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. 2014 Jun 30;9(6):e100929. doi: 10.1371/journal.pone.0100929

Figure 2. Analysis of the effects of temperature and probiotic supplementation on apoptosis in Kidney (A–C), Gill (D–F) and Liver (G–I).

Figure 2

The apoptotic cells are represented by red color while blue color represents the juxtaposed cell nucleus. Note: Different cell types i.e. nephrons, interstitial cells of kidney, primary gill lamellae, secondary gill lamellae and apoptotic hepatic cells are respectively marked with ▴, $, #, * and ∧. Bar length- 50 micron. J–L shows the relative apoptotic cell density in the kidney, gill and liver at different temperature. Each value represents Mean ±SE of three biological replicates (n = 3), each replicate prepared from four fish. Briefly, the mean data from 4 fish (from same treatment replicate) were used for calculating the biological average apoptotic cell count. The average data generated from three such biological averages (n = 3) were used for statistical analysis. Significance between the treatments was determined by Duncan's Multiple Range Test (DMRT) using SPSS (Version 16.0).