Table 1. Effect of temperature, multispecies probiotics mixture (B. subtilis, L. lactis and S. cerevisiae) and their interaction on the blood parameters of L. rohita fingerlings.
TREATMENT | NBT (A540) | Blood glucose (mg 100 ml−1) | RBC (X 106 cells µl−1) | WBC (X 103 cells µl−1) |
PROBIOTICS LEVEL | ||||
With | 0.34±0.02a | 85.26±0.47b | 1.81±0.18a | 2.49±0.21b |
Without | 0.19±0.02b | 97.16±2.48a | 0.71±0.20b | 3.03±0.12a |
P-value | 0.000 | 0.000 | 0.000 | 0.000 |
TEMPERATURE LEVEL | ||||
28°C | 0.32±0.02a | 81.49±0.23d | 1.54±0.16a | 2.46±0.08b |
31°C | 0.31±0.02a | 86.46±0.66c | 1.36±0.14ab | 2.77±0.15ab |
34°C | 0.26±0.01b | 92.87±1.89b | 1.20±0.10bc | 2.75±0.04ab |
37°C | 0.19±0.01c | 104.03±3.8a | 0.96±0.06c | 3.06±0.13a |
P-value | 0.000 | 0.000 | 0.003 | 0.010 |
TEMPERATURE X PROBIOTICS | ||||
T1 | 0.38±0.01 | 82.22±0.23d | 2.01±0.27a | 2.52±0.09c |
T2 | 0.38±0.02 | 83.93±0.11d | 1.95±0.22a | 2.53±0.14c |
T3 | 0.34±0.01 | 85.58±0.23d | 1.81±0.13a | 2.43±0.07c |
T4 | 0.26±0.01 | 89.33±0.18c | 1.48±0.11b | 2.46±0.20c |
T5 | 0.25±0.01 | 80.75±0.16e | 1.06±0.03bc | 2.39±0.33b |
T6 | 0.23±0.01 | 88.99±0.12c | 0.77±0.20cd | 3.00±0.16b |
T7 | 0.17±0.02 | 100.17±0.18b | 0.59±0.06d | 3.07±0.02b |
T8 | 0.12±0.02 | 118.73±0.25a | 0.43±0.03d | 3.66±0.02a |
P-value | 0.046 | 0.000 | 0.000 | 0.000 |
Data expressed as Mean ± SE (n = 3). Different superscripts in each column under each sub headings vary significantly (P<0.01).
T1 (28°C+BF (Basal feed) +PM (Probiotic mixture)), T2 (31°C+BF+PM), T3 (34°C+BF+PM), T4 (37°C+BF+PM), T5 (28°C+BF), T6 (31°C+BF), T7 (34°C+BF) and T8 (37°C+BF).