. 2014 Jun 30;9(6):e100929. doi: 10.1371/journal.pone.0100929

Table 2. Effect of temperature, multispecies probiotics mixture (B. subtilis, L. lactis and S. cerevisiae) and their interaction on the serum parameters of L. rohita fingerlings.

TREATMENT	Total protein (g dl⁻¹)	Albumin (g dl⁻¹)	Globulin (g dl⁻¹)	A:G ratio	ALP (IU l⁻¹)	ACP (IU l⁻¹)	HSP (nano g ml⁻¹)	MPO (A₄₅₀)	IgM (OD)
PROBIOTICS LEVEL
With	2.49±0.03^a	0.79±0.01	1.70±0.04^a	0.47±0.05^b	133.24±1.58^b	22.25±1.07^b	5.86±0.06^b	3.02±0.39^a	0.22±0.01^a
Without	2.06±0.09^b	0.77±0.02	1.28±0.08^b	0.64±0.04^a	155.90±2.11^a	33.94±0.96^a	14.70±0.41^a	0.78±0.28^b	0.10±0.02^b
P-value	0.000	0.100	0.001	0.000	0.000	0.000	0.000	0.000	0.000
TEMPERATURE LEVEL
28°C	2.47±0.05^a	0.76±0.02	1.71±0.06^a	0.44±0.02^b	130.75±3.77^c	23.56±1.55^c	5.49±1.48^d	2.54±0.27^a	0.19±0.01^a
31°C	2.39±0.07^ab	0.78±0.01	1.61±0.07^ab	0.49±0.02^ab	131.16±4.29^c	24.75±3.05^c	7.46±2.44^c	1.90±0.20^b	0.20±0.01^a
34°C	2.22±0.12^ab	0.78±0.02	1.41±0.12^ab	0.59±0.06^ab	150.97±1.91^b	29.20±0.43^b	12.04±0.03^b	1.81±0.15^b	0.14±0.01^b
37°C	2.00±0.15^b	0.81±0.02	1.23±0.14^b	0.67±0.07^a	165.40±3.27^a	34.88±1.53^a	16.13±1.37^a	1.37±0.02^c	0.12±0.00^c
P-value	0.000	0.011	0.000	0.003	0.000	0.000	0.000	0.000	0.000
TEMPERATURE X PROBIOTICS
T1	2.58±0.03^a	0.74±0.01	1.83±0.03^a	0.41±0.01^b	125.12±0.99^e	21.01±1.26^d	5.67±0.03^f	4.08±0.26^a	0.25±0.00^b
T2	2.54±0.03^a	0.75±0.01	1.77±0.03^ab	0.44±0.01^b	123.14±0.94^e	21.22±0.62^d	5.78±0.02^de	3.01±0.17^b	0.27±0.00^a
T3	2.47±0.02^a	.077±0.01	1.67±0.02^bc	0.48±0.01^b	136.12±1.83^d	22.91±0.30^d	5.93±0.02^d	2.90±0.09^b	0.20±0.01^c
T4	2.34±0.02^bc	0.78±0.02	1.54±0.04^de	0.53±0.03^b	148.57±2.97^c	23.85±0.60^d	6.07±0.14^de	2.10±0.20^c	0.17±0.01^d
T5	2.36±0.02^b	0.79±0.04	1.60±0.05^cd	0.48±0.04^b	136.37±1.02^d	26.10±1.00^c	5.31±0.07^f	1.00±0.01^d	0.13±0.00^e
T6	2.24±0.01^c	0.81±0.02	1.45±0.01^e	0.54±0.01^b	139.18±1.37^d	28.28±0.64^c	9.14±0.11^c	0.79±0.03^d	0.13±0.00^e
T7	1.96±0.03^d	0.81±0.05	1.15±0.02^f	0.71±0.06^a	165.81±1.42^b	35.49±0.53^b	18.15±0.05^b	0.71±0.02^d	0.09±0.00^f
T8	1.66±0.03^e	0.81±0.03	0.92±0.01^g	0.82±0.04^a	182.23±0.27^a	45.90±2.96^a	26.18±0.03^a	0.63±0.02^d	0.07±0.00^g
P-value	0.000	0.056	0.000	0.000	0.000	0.000	0.000	0.000	0.000

Data expressed as Mean ±SE (n = 3). Different superscripts in each column under each sub headings vary significantly (P<0.01).

T1 (28°C+BF (Basal feed) +PM (Probiotic mixture)), T2 (31°C+BF+PM), T3 (34°C+BF+PM), T4 (37°C+BF+PM), T5 (28°C+BF), T6 (31°C+BF), T7 (34°C+BF) and T8 (37°C+BF).