Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2014 Apr 24;19:135–142. doi: 10.1016/j.dnarep.2014.03.018

Figure 2. Model incision substrates for endonucleases.

Figure 2

(A–D) Preferred substrates, as determined in biochemical assays, for the indicated endonucleases. The arrowheads indicate the location of incision. Each substrate is shown in two orientations related by a 180 degree rotation. The left column shows where the nuclease would cut on the 5′ side of a converged fork; the right column shows incisions on the 3′ side. In each column, the nucleases that are likely to cut the ICL-associated X-shaped structure shown at the top are highlighted by a grey box. (E) Classic nucleotide excision repair (NER) substrate and locations of XPF and XPG incisions. (F) Location of incisions when XPF is presented with an ICL-containing fork-shaped structure. (G) Action of SNM1A on ICL-containing DNA.