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. Author manuscript; available in PMC: 2015 Mar 12.
Published in final edited form as: Adv Mater. 2013 Dec 12;26(10):1494–1533. doi: 10.1002/adma.201304431

Figure 9. Micro/nano-patterning of biochemical and biophysical cues in 3D hydrogels.

Figure 9

(a) Schematic of micro/nano-patterning in 3D hydrogels using focused laser (single-photon or two-photon). Using different photo-chemical designs of crosslinkers, one could achieve reversible biochemical patterning, mechanical modulation and multiplex immobilization of biochemical cues (see text for detailed description).[135,206,207] (b) Fluorescent confocal micrograph of 3D micro-patterns of SHH (green) and CNTF (red) within an agarose hydrogel.[207] Reproduced with permission from [207]. Copyright 2011, Nature Publishing Group. (c) Fluorescent confocal micrograph of micro-patterned 3D helices of fluorescently labeled peptides, of which one (red) was reversibly removed after UV exposure.[135] Reproduced with permission from [135]. Copyright 2012, Wiley-VCH (Germany).