Figure 4. BCL6 is transiently downregulated in CD11c^int I-A^hi subpopulation of cDCs in secondary lymphoid organs after Alum or CFA injection.

C57BL/6 mice were immunized with Alum by intraperitoneal injection or with CFA by footpad injection. (A) The average percent ±SEM (n = 4) BCL6^hi in splenic total cDCs (CD11c⁺ I-A⁺) before and after Alum injection. (B) By D2 after immunization, representative FACS data shows splenic CD11c^hi I-A^int and CD11c^intI-A^hi subpopulations (gated on single live CD19⁻ TCRβ⁻ cells), which are absent in unimmunized mice, and their CD86 expression (n = 4). CD11c^hi I-A^int (grey line) and CD11c^int I-A^hi (black line). (C) The average percent ±SEM (n = 4) BCL6^hi in splenic cDC subpopulations before and after alum injection. (D) The average BCL6 MFI ±SEM (n = 4) in splenic cDC subpopulations before and after alum injection. (E) The average BCL6 MFI ±SEM (n = 4) in LN cDC subpopulations before and after CFA footpad injection. (F) The average BCL6 MFI ±SEM (n = 4) in cDC subpopulations of mesenteric LNs under steady state. CD11c^hiI-A^int (solid bar) and CD11c^intI-A^hi (open bar); One way and two way ANOVA multiple comparasions were used (within and between cDC subpopulation), *p<0.05; **p<0.005; ***p<0.001 (ANOVA) was compared either as indicated or with their counterparts.