Figure 2. Effects of fadrozole on prolactin-positive cells in the pituitary of male rats.

a) General disposition of prolactin-positive cells in the gland. PD: pars distalis of pituitary gland. Scale bar: 100 µm. b) Irregular and strongly stained prolactin-positive cells (arrows) in untreated male rats. c) Polygonal and weakly stained prolactin-positive cells (arrows) after 5 doses of fadrozole. Scale bar b, c: 12 µm d) Plot showing the in vivo effects of the 1 or 5 doses of fadrozole on the serum levels of prolactin (ng/mL) (*p<0.01 with respect to untreated and control animals, **p<0.01, with respect to untreated and control animals and p<0.05 with respect to 1 dose of fadrozole-treated animals). Morphometric effect of treatment with fadrozole on cellular (e) or nuclear (f) areas (µm²); in both cases fadrozole induces decreases in size (*p<0.01 with respect to untreated and control animals).