Figure 4. Effects of in vitro treatment with fadrozole on prolactin-positive cells.

Double immunocytochemical staining for PCNA (brown) and prolactin (dark blue-grey) in control dishes a), testosterone-treated dishes b), and testosterone- and fadrozole-treated dishes c). Scale bar: a,b,c: 50 µm. d) Plot showing the decrease in cellular area at the different time-points assayed; from 3 to 12 hours of treatment with testosterone and fadrozole a significant decrease (*p<0.01) was observed. e) The percentage of prolactin-positive cells decreases as from 3 to 12 hours of treatment with testosterone and fadrozole (*p<0.01). f) The percentage of PCNA- and prolactin-positive cells, out of the total number of prolactin-positive cells, decreases significantly from 1 to 12 hours of treatment with testosterone and fadrozole (*p<0.01).