Fig. 2.
Dynamic 3D micropatterned co-culture in the photocurable PEG and chemically degradable alginate hydrogel. mES and HepG2 cells were encapsulated in the PEG and alginate hydrogels, respectively (Day 0). The mES and HepG2 cells were cultured for 4 days and the alginate hydrogel was subsequently degraded on day 4. (a) mES and HepG2 cells were labeled with CellTracker Green and CellTracker Red, respectively. (b) Live and dead cells were indicated by calcein-AM (green) and ethidium homodimer (red), respectively.