Fig. 4.
Morphology, gene expression, and beating characterization of mES cells through the 2nd step culture (i.e. continuous co-culture, dynamic co-culture, and monoculture). (a) Culture schedules for the continuous co-culture, dynamic co-culture, and monoculture (b) Microscope images of mES cells in the PEG hydrogel during the 2nd step culture (Day 12). (c) Cardiac gene expression in various culture conditions through the 2nd step culture. (d) Number of hydrogels containing beating colony in the three different culture conditions. Two-way ANOVA suggested significant effect of time and culture conditions on the number of beating colonies (p < 0.0001). Statistically significant differences were determined employing the Bonferroni’s multiple comparisons test and denoted as: *p<0.05 and **p<0.01. (N=5)