| DMEM w/high glucose (HyClone/Fisher Cat. # SH30022.FS) | 375 mL |
| Ham’s Nutrient Mixture F-12 (HyClone/Fisher Cat. # SH30026.01) | 125 mL |
| Fetal Bovine Serum (FBS) (MediaTech/Fisher Cat. # MT-35-011-CV) | 12.5 mL |
| 100X Hydrocortisone | 5 mL |
| 100X Cholera Toxin | 5 mL |
| 100X Adenine | 5 mL |
| 100X Epidermal Growth Factor (EGF) | 5 mL |
| 100X Insulin (made fresh) | 5 mL |
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