FIG. 1.

MicroRNA expression in PHTs cultured in standard condition (Std) and hypoxia (Hpx). A) Expression level of miR-424-5p based on read counts from high-throughput sequencing (n = 1). Cells were cultured for 48 h in normoxia or hypoxia before RNA extraction. Two libraries of small RNAs were derived from these samples and deep-sequenced using an Illumina GAII analyzer. B) Time course of miR-424 expression in PHT cells cultured for 72 h in standard conditions compared to hypoxia and based on RT-qPCR. One representative of four experiments is shown. Error bars indicate the variability between two technical replicates. Data were normalized using U6 small nuclear RNA as an endogenous control. Statistical analysis was performed based on all independent experiments. **P < 0.01 compared with the standard group. Note that miR-424 expression levels in standard conditions were also significantly (P < 0.01) elevated at 72 h compared to t = 0. C) Expression levels of miR-424 in JEG3, HUVEC, or ECFC cells. Data are mean ± SEM of three independent experiments, and each is measured in duplicate. Statistical analysis was performed on three independent experiments and is shown on a representative graph. **P < 0.01 compared with standard conditions. D) Expression levels of the miR-424-clustered miRNAs in PHT cells cultured for 72 h in standard conditions compared to hypoxia. *P < 0.05 compared with standard conditions.