FIG. 2.

Trophoblast differentiation enhances the expression of miR-424. RT-qPCR analysis of miR-424 expression in PHT cells cultured for 48 h in standard conditions (Std) or hypoxia (Hpx) and in the absence or presence of DMSO. Data were normalized using U6 small nuclear RNA as an endogenous control. Inset: the concentration of hCG released in the medium, measured by ELISA as described in Materials and Methods. Data represent the geometric mean of three independent experiments, each performed in duplicate and plotted on a logarithmic scale. *P < 0.05 and **P < 0.01 compared with the control in the standard conditions group.