FIG. 5.

Silencing of FGFR1 by members of the miR-15/16 family. A) Activity of wild-type and mutant FGFR1-3′UTR reporter genes in response to expression of members of the miR-15/16 family of miRNAs. HTR8/SVneo cells were transfected with FGFR1 reporters along with an miR-424 or miR-15/16 expression vector. RLU activity from each reporter construct, normalized to firefly luciferase activity, was determined 48 h later. Data are geometric means of three independent experiments performed in duplicate. *P < 0.05 and **P < 0.01 compared with the control group for each miRNA expression vector (FGFR1 wt). B) Expression levels of some members of the miR-424 family in PHT cells cultured for 72 h in standard conditions (Std) compared to hypoxia (Hpx). One representative of four experiments is shown, with error bars indicating the variability between two technical replicates. Data were normalized using U6 small nuclear RNA as an endogenous control.