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. 2013 Jul 10;89(2):31. doi: 10.1095/biolreprod.113.110106

FIG. 1.

FIG. 1

Differential expression of Gjb2 (gap junction protein, beta 2, connexin 26) mRNA in the peri-implantation mouse uterine LE. A) Readings of gap junction proteins from microarray analysis of D3.5 and D4.5 LE. Actb, β-actin, a housekeeping gene; *, one tenth of the readings; Hprt1, hypoxanthine phosphoribosyltransferase 1, a housekeeping gene. n = 3. Error bars represent SD. B–I) In situ hybridization of Gjb2 in D3.5 ∼ D5.5 WT (+/+) uterus and Lpar3-deficient (−/−) uterus with delayed implantation [19]. B) D3.5, +/+. C) D3.5, −/−. D) D4.5, +/+. E) D4.5, −/−. F) D5.5, +/+, enlarged view from the red box in H. G) D5.5, −/−. H) D5.5, +/+, low magnification (×4), the dotted red box and blue box shown in F and I, respectively. I) D5.5, +/+, enlarged view from the blue box in H. Bars = 100 μm (B–G and I) and 500 μm (H). Red star, embryo; GE, glandular epithelium; S, stroma; Dec, decidual zone.