FIG. 2.

Activation of the RiboTag in germ or Sertoli cells of the testis. RiboTag mice were bred with either a germ cell-specific cre line (Stra8-cre; A) or a Sertoli cell-specific cre line (Amh-cre; B). RiboTag activation was verified via immunohistochemistry using an anti-HA antibody in synchronized neonatal testis sections. Black arrows represent immunopositive spermatogonia, and purple arrows represent immunopositive Sertoli cell cytoplasm. No primary antibody negative control is represented within the inset. Bars = 50 μm. C) Microarray analysis results confirm the enrichment for well-known germ, Sertoli, Leydig, or housekeeping genes in the IP from RiboTag/Stra8-cre (top) or RiboTag/Amh-cre (bottom) mouse lines.