FIG. 4.

PCR analysis of genomic DNA from the transgenic fetuses recovered. Top panel: 538-bp EGFP amplicon. Second panel: PCR amplification product of a 443-bp region spanning the pB transposon 3′-TRE sequence and an adjacent sequence of the pBt gene outside of the transposon. Third panel: 509-bp amplicon spanning the pB transposon 5′-TRE sequence and an adjacent sequence on the plasmid backbone, outside the region of the pB transposon. Fourth panel: amplification of β-actin was used as internal loading control. MW, molecular weight marker; PC, pmGENIE-3-EGFP plasmid control used as template; WT, genomic DNA from a wild-type pig served as PCR template.