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. 2013 Oct 2;89(5):128. doi: 10.1095/biolreprod.113.110239

FIG. 4.

FIG. 4

PPP1CC2 level was drastically reduced in GcKO mice. Western blot confirming the reduced expression of PPP1CC2 in the testis of Ppp1ccΔflx/Δflx; TgStr8-Cre mice. Serially diluted testis extracts from different sets of GcKO animals were compared to that of control. a) Forty micrograms and 20 μg of total protein from different GcKO mice (except GcKO#3, where only 20 μg was used) were loaded on to each lane and compared to that of control (20 μg, 10 μg, 5.0 μg, and 2.5 μg). b) A different set of GcKO animals, using 50 and 25 μg of total protein, was compared with that of control. PPP1CC2 levels varied among knockouts but were significantly lower than that of control. c) To quantify the relative levels of PPP1CC2 in GcKO animals, we compared them with those of transgenic lines for which PPP1CC2 levels are known [18]. PPP1CC2 levels in GcKO animals were comparable to transgenic lines eTg-F10 and pTg-M3, for which PPP1CC2 levels are 12.5% that of Ppp1cc+/− animals. d) To determine the PPP1CC2 levels in brain, ∼40 μg of total protein from GcKO#1 and 2 and control brain extracts were loaded. It is to be noted that PPP1CC2 levels remained unchanged in GcKO and control brain extracts, further confirming the testis-specific deletion of the Ppp1cc gene.