FIG. 8.

OVA⁺ implantation sites contain more OT-I T cells. Cells from pooled healthy implantation (IS) or resorbing (RS) placentas of individual B6-bred or OVA-bred OT-I mice at gd10.5 were counted then stained with antibodies to CD4, CD8, and ICOS and analyzed by flow cytometry. A) Total cellularity per implantation site. B) Mean percentage of total cells that are CD4⁻CD8⁺. C) Mean percentage of CD4⁻CD8⁺ cells that are ICOS⁺. D) Mean number of CD8⁺ cells per implantation site. SEM is shown and significant differences were determined using a Student t-test (*P < 0.05, **P < 0.005 between B6-bred and OVA-bred mice). For B6-bred, OVA-bred IS, and OVA-bred RS, n = 6, 5, and 5, respectively.