FIG. 3.

Short-hairpin RNA-mediated Lin28a KD in mTS cells results in increased levels of let-7 miRNA. A) LIN28A protein levels in Lin28a KD and nontarget control mTS cells (columns 1–4) in relation to mTS cells at Day 0 and after 6 days in differentiation medium (columns 5–8). B) Densitometry analysis of LIN28A protein bands (calculations made as a percent of GAPDH protein). C) Levels of let-7 miRNA in Lin28a KD and nontarget control mTS cells, and mTS cells collected at Day 0 and after 6 days in differentiation medium. Error bars represent SEM. Different letters indicate P < 0.05.