FIG. 2.

Testis sections from Foxp3^+/Y (A) and Foxp3^sf/Y (B) mice were stained with PASH, revealing that seminiferous tubule diameter is reduced in Foxp3^sf/Y mice and spermatogenesis is arrested. Immunohistochemistry for HSD3B1 was used to label Leydig cells in testis from Foxp3^+/Y (C and E) and Foxp3^sf/Y (D and F) mice. Foxp3^sf/Y mice exhibit smaller clusters of Leydig cells than their wild-type littermates. Four animals were analyzed per genotype. Original magnifications ×400 (A and B), ×630 (C and D), and ×100 (E and F); bar = 100 μm.