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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Cell. 2014 Jan 16;156(0):317–331. doi: 10.1016/j.cell.2013.12.010

Figure 2. GSH Depletion Inactivates GPX Enzymes to Induce Ferroptosis.

Figure 2

(A) Basal ROS levels among BJ-derived cell lines were compared (n = 8).

(B) The growth inhibition effect of antioxidant-targeting compounds was determined in the four BJ-derived cells (n = 3). The bar graph indicates growth inhibition at two different concentrations (2× GI50 and 4× GI50 for each compound in BJeLR cells).

(C) Other antioxidant inhibitors do not deplete GSH during cell death (n = 3).

(D) Erastin depletes cellular GSH equally in the four BJ-derived cell lines.

(E) GSH-depleting reagents elevated both cytosolic and lipid ROS level, whereas other antioxidant inhibitors did not (n = 3).

(F) GSH-depleting reagents (ERA and BSO) inhibited GPX activity. Rel. Abs., relative absorbance.

n.s., not significant; *p < 0.05; ***p < 0.001. Error bars in (B), (C), and (E) represent mean ± SD. See also Figure S2.