Fig. 1.

Establishment of in vivo model of renal ischemia-reperfusion injury (IRI). (A) FK506 pre-conditioning was performed 30 minutes before surgery. Renal pedicles were occluded by clamping for 25 minutes, and reperfusion followed by removing these clips. Arrowheads indicate the color change in the kidney from renal ischemia/reperfusion (I/R) mice. One day later, the cross section of the IRI kidney revealed hemorrhagic infarcts in the outer medulla. (B) Creatinine levels were measured in serum from I/R or FK506-preconditioned I/R mice. Creatinine levels in I/R mice increased to more than 5-fold those in the sham control (CTL) mice. FK506 reduced these levels, which was statistically significant at 1 µg/kg. All experiments were performed in triplicate and repeated twice. ^*P<0.01, ^***P<0.0001.