Fig. 4.

FK506 reduced pro-inflammatory cytokine expression and neutrophil infiltration. Tumor necrosis factor-α (TNF-α) expression was strong in the tubules around glomeruli, where calcineurin expression was high, as shown in Fig. 2A. FK506 reduced TNF-α expression in the distal tubules in the cortex and the tubules in the outer medulla. I/R, ischemia/reperfusion; HPF, high power field. (B) Expression of Ly6B.2, a neutrophil infiltration marker, was observed at high levels in the outer medulla and particularly in the interstitium. Levels of Ly6B.2 were significantly reduced by FK506 treatment. (C) Expression of intercellular adhesion molecule-1 (ICAM-1) was observed mostly in the tubules and interstitium. FK506 decreased ICAM-1 to sham control levels. All experiments were performed in triplicate and repeated twice. ^*P<0.01, ^**P<0.001, ^***P<0.0001. Scale bars=100 µm (A-C).