Figure 1.

The scheme depicts the protein and gene structure of RS2Z33 and its transcripts. A) The Molecular Beacon was designed to bind to exon junction 6 of RS2Z33; i.e. all splice variants of RS2Z33 are targeted. Red – intronic sequences; Black – exonic sequences; white – untranslated regions; RRM – RNA recognition motif; ZnK – Zink knuckle; SR – Serine/Arginine rich domain; SP – Serine/Proline-rich domain; alt 3’-ss – alternative 3’ splice site; IR – intron retention; I2 – intron 2; MB – Molecular Beacon; EJ6 – exon junction 6; F – fluorophore; Q – quencher. B) Illustration of the work principle of MB.