Figure 4.

Effects of alcohol and betaine on hepatic colocalization of SR-B1 with Na⁺/K⁺ ATPase1. Six rats per group were pair fed their respective HFC, HFE, and HFEB liquid diets for 8 weeks, after which the liver sections were obtained and stained for SR-B1 and Na⁺/K⁺ ATPase1, as described in Materials and Methods. A: Representative images of the individual channels for SR-B1 (green), Na⁺/K⁺ ATPase1 (red), nucleus (blue), and the merged channels, indicating regions of colocalization in yellow. B: The co-occurrence and quantification of SR-B1 with the plasma membrane marker protein, Na⁺/K⁺ ATPase1, is expressed as the average percentage of pixels of SR-B1 relative to the pixels of Na⁺/K⁺ ATPase1. C: Pearson's correlation coefficient was calculated to describe the colocalization of the intensity distribution between SR-B1 and Na⁺/K⁺ ATPase1. Data are expressed as means ± SD in each group. Statistical significance of variance was calculated using Tukey's test: ^∗P < 0.05 versus HFC, ^†P < 0.05 versus HFE. Scale bar = 20 μm (A).