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. 2014 Jul;184(7):1930–1939. doi: 10.1016/j.ajpath.2014.03.011

Figure 4.

Figure 4

ER expression is comparable in mouse and human lesions. A: Quantitative RT-PCR analysis of ERα (black bars) and ERβ (white bars) in the mouse uterus (n = 6), peritoneum (n = 6), and endometriotic (Endo) lesions (n = 6). B: Quantitative RT-PCR analysis of ERα and ERβ in human endometrium (Endo) (n = 18), peritoneum (n = 19), and peritoneal lesions (n = 19). Quantitative RT-PCR data were analyzed using a one-way analysis of variance and the Newman-Keuls postcomparison test. C: Diaminobenzidine (DAB) IHC analysis using an anti-ERα antibody on mouse lesions revealed moderate immunoreactivity in glandular epithelium. D: DAB IHC analysis using an anti-ERα antibody on human lesions also revealed moderate immunoreactivity in epithelium and stromal areas. Insets in C and D show negative controls with omission of primary antibody. E: DAB IHC analysis using an anti-ERβ antibody on mouse lesions reveals prominent ERβ immunoreactivity in glandular epithelium and stromal regions. F: DAB IHC analysis using an anti-ERβ antibody on human lesions also reveals strong immunoreactivity in glandular and stromal regions. IHC analysis of human lesions was performed on samples dissected during the proliferative (estrogen-dominated) stage of the menstrual cycle. Data are expressed as means ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. RQ, relative quantification.