Figure 4.

Blood vessels and myofibroblasts in wound areas are reduced in MFG-E8 KO mice. A: Distribution of ECs (CD31⁺) and pericytes (NG2⁺) in the granulation tissue in the wound in MFG-E8 WT and KO C57BL/6 mice at 7 days after wounding. B: Distribution of αSMA⁺ pericytes or myofibroblasts in the dermis of wounds in MFG-E8 WT/KO C57BL/6 mice at 7 days after wounding. C: Distribution of CD68⁺ macrophages in the dermis of wounds in MFG-E8 WT/KO C57BL/6 mice at 7 days after wounding. A–C: Quantification of the CD31⁺, NG2⁺, αSMA⁺, and CD68⁺ areas in eight random microscopic fields was performed using ImageJ software. Positive areas in WT mice were assigned values of 1. The dotted lines indicate the border between the epidermis and the dermis. Data are expressed as means ± SEM. n = 3 mice per genotype. ^∗∗P < 0.01, Student's t-test. Scale bar = 20 μm.