Figure 6.

Accumulation and distribution of MFG-E8 in close proximity to pericytes in human granulation tissue. A: Clinical appearance of normal granulation tissue in decubitus ulcer and fibrous granulation tissue in intractable decubitus ulcer. B: Histological appearance of normal and fibrous human granulation tissue. H&E stain. C: Localization of MFG-E8 in relationship to endothelial cells (CD31⁺) or pericytes (αSMA⁺) in human granulation tissue. D: Quantification of the extent of MFG-E8 expression, CD31⁺ ECs, and αSMA⁺ pericytes in normal and fibrous granulation tissue in eight random microscopic fields in three independent experiments was performed using ImageJ software. Positive area in normal granulation tissue was assigned a value of 1. Data are expressed as means ± SEM. n = 3. ^∗∗P < 0.01, Student's t-test. Scale bar = 20 μm.