Figure 2.

IL-19^−/− mice are not Th1 polarized. Spleen removed from age-matched WT and IL-19^−/− littermates 28 days after carotid ligation; mRNA was extracted and reverse transcribed, and amplified using the primer pairs listed in Materials and Methods. No significant difference was noted in mRNA abundance for T-bet (A) or GATA3 (B) (n = 8 spleens in each group). C: Lack of IL-19 results in increased leukocyte infiltrate in ligated arteries. Serial sections were immunostained using anti-CD45 antibody. Positive cells were counted from at least four stained tissue sections from four different mice, and values expressed are positive cells per HPF. D: IL-19 does not increase CAM expression in monocytes. THP1 cells were treated with rIL-19, and CAM mRNA expression was quantitated by quantitative RT-PCR. There is no significant difference in mRNA expression between IL-19 and untreated THP1 cells. ^∗P < 0.05.