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. 2014 Jul;184(7):2134–2143. doi: 10.1016/j.ajpath.2014.04.001

Figure 4.

Figure 4

IL-19 regulates VSMC migration, but not VSMC phenotype. A: IL-19 does not modify VSMC phenotype. WT or IL-19−/− VSMCs were serum starved, then stimulated with FCS. SMC phenotype marker expression, quantitated by quantitative RT-PCR, indicates no significant difference between WT and IL-19−/− VSMCs for any marker. B: WT or IL-19−/− VSMCs were seeded onto the top chamber of a modified Boyden chamber in medium containing 0.2% bovine serum albumin, with or without 40 ng/mL PDGF as a chemoattractant in the lower chamber. Values are means from three experiments performed in triplicate from three independent groups of VSMCs. IL-19 reduces VSMC wound healing. C: Scratch wound migration of IL-19−/− VSMCs. Cells were stained with hematoxylin. Dashed lines define original scratched area. Photomicrograph is representative of three independent experiments. Quantitative analysis of scratch wound area is from three different groups of treated VSMCs. ∗P < 0.05, ∗∗∗P < 0.001 versus control. Original magnification, ×40.