Figure 3.

Functional characterization of LLC1 tumor cells. A) qPCR was performed to analyze the expression of the PAF-R in LLC1 tumor cells. PAF-R deficient murine B16F10 and ectopic PAF-R expressing B16-PAFR melanoma cells were used as negative and positive controls. In addition, ectopic and endogenous PAF-R expressing human KBP and SK23mel cells were used as positive controls. B) Intracellular calcium (Ca²⁺) assay was performed with LLC1 cells treated with 1 μM CPAF. LLC1 cells treated with 1 μM SLIGRL, a protease activated receptor (PAR) agonist was used as a positive control. C) Cell proliferation assay was performed in LLC1 tumor cells treated either with vehicle (0.1% ethanol) or CPAF and cultured for 24 and 48 hours. After each time points, cells were trypsinized, stained with trypan blue and counted by digital cell counter.