Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul;144(1):81–104. doi: 10.1085/jgp.201411169

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Xu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Design of mP2X4 receptors carrying pHluorin in the extracellular domain. (A) Cartoons illustrate the basic layout of a P2X receptor subunit and how three identical subunits are assembled into a trimeric ion channel. The location of the PM is shown approximately with black lines. (B) Sequence alignment for parts of the extracellular loop that were targeted for insertion of pHluorin. The pHluorin insertion sites are shown with a dashed line and a cartoon of GFP. Nine constructs were made and tested using approaches described in detail in the Results. They are identified in the figure.