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. 2014 Jul;144(1):81–104. doi: 10.1085/jgp.201411169

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© 2014 Xu et al.

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Figure 6. — P2X4-pHluorin123 receptors display pH-dependent fluorescence changes. (A) Representative images of a HEK-293 cell expressing P2X4-pHluorin123 at the indicated pH values. (B) Average traces for pH-dependent changes in the fluorescence of HEK-293 cells expressing P2X4-pHluorin123. (C) Summary data for pH-dependent changes in the fluorescence of P2X4-pHluorin123. The pKa value was ∼7.3, which is similar to past characterization work on pHluorin (see Results).