Table 1.

Primers used to generate mP2X4 constructs carrying pHluorin

Primer	Sequence (5′ to 3′)
P2X4 extreme flanking primers
P2X4 extreme 5′	AGTTAAGCTTGGTACCGAGCTCGGATCC
P2X4 extreme 3′	TATGCGGCCGCTCACTTGTCATCGTCATCCTTGTAATCCTGGTCCGTCTCTCCGGAAAG
P2X4 insertion site primers
P2X4-pHluorin79 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCCTGAGAAGTGTTGGTCACAGCCACACCTTTGGCTTTGG
P2X4-pHluorin79 B-5′	GGAGCTGGAGCTATCGATTATGAATTCGCTGGAGCTGGACTTGGATTCCGGATCTGGGACGTGGCCGACTATGTGG
P2X4-pHluorin82 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCGAATCCAAGCTGAGAAGTGTTGGTCAC
P2X4-pHluorin82 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGACGGATCTGGGACGTGGCCGACTATG
P2X4-pHluorin123 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCCTTATCAGGAATCTCTGGACAGGTACC
P2X4-pHluorin123 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGAACCAGCATTTGCGATTCAGACGCCAAC
P2X4-pHluorin156 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCAGACGCGTTGAATGGAACACACCTTCC
P2X4-pHluorin156 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGAGTGAAGACCTGTGAGGTGGCCGCATGG
P2X4-pHluorin207 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCGAGGATATTCCTCTTGCTGAAATTAAAC
P2X4-pHluorin207 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGACCCAACATTACCACCTCCTACCTCAAG
P2X4-pHluorin222 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCCGCATTATAAATGCACGACTTGAGG
P2X4-pHluorin222 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGACGGACGGATCCCTTCTGCCCCATATTCC
P2X4-pHluorin224 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCCGTCCGCGCATTATAAATGCACGAC
P2X4-pHluorin224 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGAGATCCCTTCTGCCCCATATTCCGTC
P2X4-pHluorin304 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCAAGGTCCCTGTAGTACTTGGCAAACC
P2X4-pHluorin304 B-5′	GGAGCTATCGATTATGAATTCGCTGGAGCTGGAGCTGGCAATGAGCAACGCACACTCACC
P2X4-pHluorin307 A-3′	TCCAGCGAATTCATAATCGATAGCTCCAGCTCCAGACGCGTTGAATGGAACACACCTTCC
P2X4-pHluorin307 B-5′	GAGCTATCGATTATGAATTCGCTGGAGCTGGAGAGCAACGCACACTCACCAAGGCATATG
pHluorin amplification primers
pHluorin Cla1 5′	TATATCGATGGAGGAGCTGGAGGAGCTAGGAGTAAAGGAGAAGAACTTTTCACTGG
pHluorin EcoR1 3′	ATAGAATTCAGCTCCTCCAGCTCCTCCTTTGTATAGTTCATCCATGCCATGTGTAATCC