Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul;144(1):115–124. doi: 10.1085/jgp.201411179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Yu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Measuring bi-ionic potential of the TMEM16A current. (A) Activation of Cl⁻ current (top) in an excised inside-out patch expressing TMEM16A in symmetrical 140 mM [Cl⁻]. (Bottom) The digital signal used to switch between the solutions containing zero-Ca²⁺ and 20 µM of free [Ca²⁺]. (B) Current-clamp (I = 0) recordings to monitor the change of bi-ionic potentials upon the switch of anion solutions on the intracellular side. Intracellular solutions contained 20 µM of free [Ca²⁺] throughout the recording. Same patch as in A. (Bottom) The digital signal for triggering solution exchange. (C) Bi-ionic potentials measured by voltage-clamp recordings. The two panels were recordings from two different patches consisting of currents of different amplitudes (see the scale of the y axis). Different values of E_rev were obtained as exemplified in the bi-ionic condition of SCN⁻/Cl⁻. (D) The SCN⁻/Cl⁻ bi-ionic potential as a function of the current amplitude at 40 mV in symmetrical 140 mM Cl⁻. Each data point was from an independent patch measured either by the voltage-clamp or by the current-clamp recording.