Table 1.
Details of the Primary Antibodies Used in This Study
| Antibody | Source and type | Company | Dilution |
|---|---|---|---|
| ABCA1 | Rabbit polyclonal | Novus Biologicals (Littleton, CO) | 1:1000 |
| Calregulin (H-170) | Rabbit polyclonal | Santa Cruz Biotech | 1:500 |
| Caspase-3 | Rabbit polyclonal | Cell Signaling (Danvers, MA) | 1:1000 |
| Caspase-12 | Rat monoclonal | Sigma-Aldrich | 1:500 |
| CHOP | Mouse monoclonal | Cell Signaling | 1:1000 |
| COX IV | Rabbit polyclonal | Cell Signaling | 1:1000 |
| Cytochrome c (clone 7H8.2C12) | Mouse monoclonal | Santa Cruz Biotech | 1:500 |
| Phospho-eIF2α | Rabbit polyclonal | Invitrogen | 1:1000 |
| eIF2α | Mouse monoclonal | Abcam | 1:1000 |
| GAPDH (6C5) | Mouse monoclonal | Santa Cruz Biotech | 1:5000 |
| GRP78 | Rabbit polyclonal | Stressgen (Enzo Life Science) | 1:1000 |
| HXK I (G-1) | Mouse monoclonal | Santa Cruz Biotech | 1:200 |
| HMG-CoA reductase | Goat polyclonal | Santa Cruz Biotech | 1:500 |
| INSIG-1 | Rabbit polyclonal | Abcam | 1:1000 |
| MAP2 | Mouse monoclonal | Sigma-Aldrich | 1:500 |
| MFN2 | Mouse monoclonal | Abcam | 1:1000 |
| StARD3 | Rabbit polyclonal | Abcam | 1:1000 |
| Na+,K+ATPase α1 | Mouse monoclonal | Santa Cruz Biotech | 1:500 |
| PACS-2 | Goat polyclonal | Santa Cruz Biotech | 1:200 |
| Phospho-PERK (Thr980) | Rabbit polyclonal | Cell Signaling | 1:1000 |
| PERK | Rabbit polyclonal | Sigma-Aldrich | 1:1000 |
| Rab5A | Rabbit polyclonal | Santa Cruz Biotech | 1:250 |
| Rab7 | Goat polyclonal | Santa Cruz Biotech | 1:250 |
| Sigma receptor (B-5) | Mouse monoclonal | Santa Cruz Biotech | 1:200 |
| SREBP-2 | Rabbit polyclonal | Abcam | 1:1000 |
| StAR | Mouse monoclonal | Abcam | 1:1000 |
| VDAC | Rabbit polyclonal | Calbiochem (EMD Millipore) | 1:500 |
| β-actin | Mouse monoclonal | Sigma-Aldrich | 1:10,000 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MFN2, mitofusin-2; PACS-2, phosphofurin acidic cluster sorting protein 2; VDAC, voltage-dependent anion-selective channel protein.