Figure 3.

Impaired contraction in Bcl2l11^−/− NK cells reflects decreased apoptosis. (A) The percentages of adoptively transferred Bcl2l11^−/− or WT BrdU⁺NK1.1⁺TCRβ⁻Ly49H⁺ NK cells or BrdU⁺TCRβ⁺CD8⁺ T cells are plotted for infected and uninfected mice at day 11 after MCMV, after a 2-h in vivo pulse of BrdU. Representative flow cytometry plots are shown for CD45.2⁺ (Bcl2l11^−/−) and CD45.2⁻ (WT) cells (n = 3 mice per time point). (B) The percentages of Ki67⁺NK1.1⁺TCRβ⁻Ly49H⁺ NK cells or Ki67⁺TCRβ⁺ T cells are shown for infected and uninfected mice at day 11. Representative flow cytometry plots are shown for CD45.2⁺ (Bcl2l11^−/−) and CD45.2⁻ (WT) cells. (C and D) The percentage of annexin V⁺ (C) and FLICA⁺ (D) NK1.1⁺TCRβ⁻Ly49H⁺ NK cells is shown for the Bcl2l11^−/− and WT NK cell subsets in infected and uninfected mice at day 15 after MCMV. TCRβ⁺ T cells are shown as a control (n = 3 mice per group). Data in each panel are representative of two to three independent experiments. Error bars signify the standard error of the mean. **, P < 0.01; *, P < 0.05.