Fig. 1.

Trx1 (a), RocF (b), and AhpC (c) expression of H. pylori in gastric diseases (gastritis, peptic ulcer, and gastric cancer) evaluated by real-time PCR

The mRNA was extracted from H. pylori strains isolated from 14 gastritis samples, 13 peptic ulcer samples, and 13 gastric cancer samples. The Trx1 (a), RocF (b), and AhpC (c) expression of H. pylori was evaluated in these tissues by real-time PCR and normalized to the control gene 16S rRNA. The ΔC _T value of each sample was calculated as C _{T,target gene}−C _{T,16S rRNA}. We successfully detected Trx1 and AhpC in all 40 strains and RocF in 33 strains out of 40. ^* When compared with tissues displaying gastritis, P<0.05 was considered statistically significant. ^# When compared with peptic ulcer samples, P<0.05 was considered statistically significant