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. 2014 May 8;5(3):66. doi: 10.1186/scrt455

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Copyright © 2014 Zhang and Dou; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Isolation and identification of bone marrow mesenchymal stem cells of human first-trimester abortus. Adhered spindle cells appeared in culture of whole bone marrow (A) and they clonally grew and spread at the bottom of a culture dish within the 12th day (B). Flow cytometric analysis showed that passage 6 of the isolated cells strongly expressed the surface markers of mesenchymal stem cells, such as CD29, CD44 and CD166, but almost no markers of Hematopoietic Stem Cells, such as CD11a, CD14 and CD34 (C). FITC, fluorescein isothiocyanate; PE, phycoerythrin.