Figure 4.

Immunofluorescence assay of whole islet-like cell clusters. The strongest expression of nestin is in islet-like cell clusters after the first-stage induction in the nonadherent induction group (A), (B), (C) and the adherent induction group (D), (E), (F). The strongest expression of insulin and c-peptide is in islet-like cell clusters after the third-stage induction in the nonadherent induction group (G), (H), (I), (J) and the adherent induction group (K), (L), (M), (N). Non-induced bone marrow mesenchymal stem cells of human first-trimester abortus were negative for insulin and c-peptide (O), (P), (Q), (R).